Biochemistry

Topics Distribution:

UNIT I – Biomolecules & Bioenergetics

Biomolecules

  • Intro, classification, chemical nature & role of carbohydrates, lipids, nucleic acids, amino acids, proteins.

Bioenergetics

  • Free energy, endergonic/exergonic reactions.

  • Free energy–enthalpy–entropy relation.

  • Redox potential.

  • Energy-rich compounds: ATP, cyclic AMP.


UNIT II – Carbohydrate Metabolism & Biological Oxidation

Carbohydrate Metabolism

  • Glycolysis: pathway, energetics, significance.

  • Citric Acid Cycle: pathway, energetics, significance.

  • HMP shunt, G6PD deficiency.

  • Glycogen metabolism, GSD.

  • Gluconeogenesis: pathway, significance.

  • Hormonal regulation of blood glucose, diabetes mellitus.

Biological Oxidation

  • Electron Transport Chain (ETC) mechanism.

  • Oxidative & substrate phosphorylation.

  • Inhibitors/uncouplers.


UNIT III – Lipid & Amino Acid Metabolism

Lipid Metabolism

  • β-oxidation (Palmitic acid).

  • Ketone bodies, ketoacidosis.

  • Fatty acid synthesis.

  • Cholesterol: significance, conversion to bile acids, steroid hormones, vitamin D.

  • Disorders: hypercholesterolemia, atherosclerosis, fatty liver, obesity.

Amino Acid Metabolism

  • Transamination, deamination, decarboxylation.

  • Urea cycle, disorders.

  • Phenylalanine/tyrosine catabolism, related disorders.

  • Biological amines: 5-HT, melatonin, dopamine, noradrenaline, adrenaline.

  • Heme catabolism, hyperbilirubinemia, jaundice.


UNIT IV – Nucleic Acid Metabolism & Genetic Information Transfer

Nucleic Acid Metabolism

  • Purine & pyrimidine biosynthesis.

  • Purine catabolism, hyperuricemia, gout.

Genetic Information Transfer

  • Genome organization.

  • DNA/RNA structure & function.

  • DNA replication.

  • Transcription.

  • Genetic code.

  • Translation & inhibitors.


UNIT V – Enzymes

  • Intro, properties, nomenclature, IUB classification.

  • Enzyme kinetics: Michaelis plot, Lineweaver–Burke plot.

  • Enzyme inhibitors: types, examples.

  • Regulation: induction, repression, allosteric.

  • Applications: therapeutic, diagnostic, isoenzymes.

  • Coenzymes: structure, functions.

Coenzymes: Structure and Biochemical Functions

Coenzymes are small, organic molecules that bind to enzymes and are essential for their catalytic activity. Derived from vitamins, they…

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Therapeutic Applications of Enzymes and Isoenzymes

Therapeutic Applications of Enzymes and Isoenzymes refer to the use of naturally occurring or engineered enzymes and their isoforms (isoenzymes)…

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Regulation of enzymes

Regulation of enzymes through various mechanisms to ensure that biochemical reactions occur at the appropriate times and rates within a cell.…

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Enzyme Inhibitors

Enzyme inhibitors are molecules that interact with enzymes to regulate their activities. They are vital for cellular control and pharmaceutical…

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Lineweaver-Burk Plot Enzyme Kinetics

The Lineweaver-Burk plot, also known as the double-reciprocal plot, is a graphical representation used to determine important kinetic parameters of…

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Enzyme Kinetics: Michaelis-Menten Plot

Enzyme kinetics is the study of the rates of enzyme-catalyzed reactions. One of the most fundamental models in enzyme kinetics…

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Nomenclature and IUBMB Classification of Enzymes

The nomenclature and IUBMB classification of enzymes refers to the systematic method used to name and categorize enzymes based on…

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Enzymes Properties

Enzymes Properties Definition Enzymes Properties: It is biological catalysts, typically proteins, that speed up the rate of chemical reactions in…

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Oxidative Phosphorylation & Uncouplers

Oxidative phosphorylation is a key process in cellular respiration that occurs in the inner mitochondrial membrane, producing most of the…

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Enzymes Introduction

Enzymes Introduction Enzymes Introduction: It is biological catalysts that speed up chemical reactions in living organisms. They are typically proteins,…

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