Growth Conditions in plant tissue culture

• Growth Conditions in plant tissue culture involve light, temperature, humidity, and sterile environment control.
• Growth Conditions in plant tissue culture ensure optimal cell division, differentiation, and healthy plantlet development.

Sterile Environment

• All culture media, instruments, and vessels must be sterilized (commonly by autoclaving at 121°C, 15 psi, 15–20 min).
• Explants surface-sterilized (e.g., with sodium hypochlorite or ethanol) to remove contaminants.

Temperature

• Most tissue cultures thrive at 25–28°C.
• Some species require slightly different temperatures for optimal growth.

Light

• Photoperiod typically: 16-hour light / 8-hour dark cycle.
• Light intensity and quality (fluorescent lamps, LEDs) can influence morphogenesis (shoot vs. root induction).

Humidity

• High relative humidity (>70
• Prevents desiccation of exposed tissues and supports healthy plantlet growth.

pH

• Typically adjusted to 6–5.8 before autoclaving.
• Ensures nutrient availability and stability of the gel matrix.

Thank you for reading from Firsthope's notes, don't forget to check YouTube videos!