Pharmaceutical Microbiology Notes (S-3)
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Unit 1
Introduction to Microbiology and Bacterial Cultivation Techniques
Unit I covers microbiology basics, history, scope, and types of microorganisms. It includes bacterial structure, growth, culture methods, and microscopy techniques like phase contrast, dark field, and electron microscopy.
Unit 2
Sterilization Techniques and Staining Methods
Unit II focuses on bacterial identification using staining techniques (simple, Gram’s, acid-fast) and IMViC biochemical tests. It also covers sterilization methods (physical, chemical, radiation), their evaluation, large-scale equipment, and sterility indicators.
Unit 3
Fungi, Viruses, and Sterility
Unit III covers morphology, classification, reproduction, and cultivation of fungi and viruses. It includes disinfectant types, modes of action, evaluation of antiseptics, and sterility testing as per IP, BP, and USP standards.
Unit 4
Aseptic Area and Assay Methods
Unit IV covers aseptic area design, laminar flow equipment, contamination sources and control, cleanroom classification, microbiological assays, and standardization and assessment of antibiotics, vitamins, and amino acids.
Unit 5
Microbial Spoilage & Cell Culture
Unit V covers types and factors of microbial spoilage in pharmaceuticals, sources of contamination, preservation using antimicrobials, and evaluation of microbial stability. It also includes animal cell culture methods and applications in industry and research.
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