Genetic Engineering & rDNA Technology Notes [U-2]

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Cloning Vector & Genetic Engineering

  • Cloning vector
  • Restriction Endonucleases (Restriction Enzymes)
  • DNA ligase
  • Recombinant DNA Technology
  • Application of Genetic Engineering in Medicine
  • Production of Interferon Using rDNA Technology
  • Production of Hepatitis B Vaccine Using rDNA Technology
  • Production of Insulin Using rDNA Technology
  • Polymerase Chain Reaction (PCR)
  • Polymerase Chain Reaction (PCR)
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Other Units of Pharmaceutical Biotechnology

Unit 1
Unit 3
Unit 4
Unit 5
Pharmaceutical Biotechnology
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Other Subjects of B Pharmacy 6th Semester

Herbal Drug Technology

Topic wise notes of:

Herbal Drug Technology

  • Herbal Raw Materials & Indian Medicine Systems
  • Nutraceuticals: Uses, Herbs & Interactions
  • Herbal Cosmetics, Excipients & Formulations
  • Herbal Drug Evaluation, Patents & Regulations
  • Herbal Industry: Scope, GMP & Infrastructure
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Absorption & Distribution

Topic wise notes of:

Biopharmaceutics and Pharmacokinetics

  • Biopharmaceutics: Absorption & Distribution
  • Drug Elimination, Bioavailability & Bioequivalence
  • Pharmacokinetics Models, Dosing & Parameters
  • Multicompartment models
  • Nonlinear Pharmacokinetics
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Pharmacology III Respiratory and GI Tract

Topic wise notes of:

Pharmacology III

  • Respiratory & GI Tract Drug Pharmacology
  • Chemotherapy: Principles & Key Antibiotics
  • Chemotherapy: TB, Fungal & Parasitic Drugs
  • UTIs, Cancer Chemo & Immunopharmacology
  • Toxicology Basics & Chrono pharmacology
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Antibiotics & Aminoglycosides in Medicinal Chemistry III

Topic wise notes of:

Medicinal Chemistry III

  • Antibiotics & Aminoglycosides
  • Antibiotics & Antimalarials: Structures & SAR
  • Anti-TB, UTI & Antiviral Agents
  • Antifungals, Antiprotozoals & Sulfonamides
  • Intro to Drug Design & QSAR Approaches
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Pharmaceutical Quality Assurance

Topic wise notes of:

Pharmaceutical Quality Assurance

  • Quality Assurance, GMP, ICH, QbD & Quality System
  • GMP: Personnel, Premises & Equipment Control
  • Quality Control and Good Laboratory Practice
  • Complaints & Document Management
  • Calibration and Warehousing
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Unit II Summary: Genetic Engineering and Applications Notes

  1. Cloning Vectors & Enzymes:
    • Cloning vectors (e.g., plasmids, bacteriophages) carry foreign DNA into host cells.
    • Restriction endonucleases cut DNA at specific sequences, aiding gene insertion.
    • DNA ligase joins DNA fragments, forming recombinant molecules.
  2. Recombinant DNA Technology:
    • Combines DNA from different sources to create genetically modified organisms.
    • Used for gene cloning, protein production, and therapy.
  3. Medical Applications:
    • Genetic engineering helps produce therapeutic proteins and diagnose diseases.
    • Offers tools for gene therapy and monoclonal antibody development.
  4. rDNA Technology in Bioproduction:
    • Interferons: Produced to boost immune response against viruses.
    • Vaccines (Hepatitis B): Genetically engineered yeast cells produce viral proteins.
    • Insulin: Human insulin gene inserted into bacteria for mass production.
  5. Polymerase Chain Reaction (PCR):
    • Amplifies specific DNA sequences rapidly.
    • Essential for diagnostics, forensics, and research

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