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Bioassay of 5-HT (Serotonin)

Updated: Jan 20

Principle of Bioassay of 5-HT (Serotonin)

  • 5-HT (Serotonin) also causes smooth muscle contraction in certain tissues (e.g., rat fundus strip, guinea pig ileum).

  • The bioassay measures the contractile response to 5-HT in an isolated tissue preparation. (Bioassay of 5-HT) (Serotonin)

Methods

Rat Fundus Strip or Guinea Pig Ileum

Procedure:

  • Mount the tissue in an organ bath.

  • Apply standard 5-HT in graded doses, record the contractile response. Wash out between doses.

  • Repeat with the test preparation.

  • Potency Determination: Compare dose-response curves, typically focusing on the EC50 or the dose producing a specific level of contraction (e.g., 50% maximal).


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