Bioassay of 5-HT (Serotonin)

• Bioassay of 5-HT (Serotonin): Commonly done using rat fundus strip or uterus for serotonin activity.
• Bioassay of 5-HT (Serotonin): Measures potency and action via smooth muscle contraction response.

Principle

• 5-HT (Serotonin) also causes smooth muscle contraction in certain tissues (e.g., rat fundus strip, guinea pig ileum).
• The bioassay measures the contractile response to 5-HT in an isolated tissue preparation.

Methods

1. Rat Fundus Strip or Guinea Pig Ileum

• Procedure:
  1. Mount the tissue in an organ bath.
  2. Apply standard 5-HT in graded doses, record the contractile response. Wash out between doses.
  3. Repeat with the test preparation.
• Potency Determination: Compare dose-response curves, typically focusing on the EC50 or the dose producing a specific level of contraction (e.g., 50

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