Growth of Animal cells in Culture involves growing animal cells outside their natural environment under controlled laboratory conditions. This process allows for the study of cell biology, physiology, and biochemistry in a controlled setting, facilitating research and development in various biomedical fields. General Procedure for Growth of Animal Cells in Culture 1. Preparation of Culture … Read more
Evaluating of microbial stability of formulations is crucial to ensure that the product remains safe and effective throughout its shelf life. This involves several testing methods and standards: 1. Microbial Limit Tests (MLT) for Evaluation of Microbial Stability of Formulations: Purpose: Determine the number of viable aerobic microorganisms in non-sterile products. Methods: Total Aerobic Microbial … Read more
Preservation of Pharmaceutical Products Using Antimicrobial Agents, particularly those that are aqueous or semi-solid, are susceptible to microbial contamination, which can compromise their safety and efficacy. To prevent this, antimicrobial preservatives are commonly added to formulations. Types of Antimicrobial Agents for Preservation of Pharmaceutical Products Preservatives: Parabens (Methylparaben, Propylparaben): Widely used due to their broad-spectrum … Read more
Regular assessment of Microbial Contamination and Spoilage is essential to detect contamination early and take corrective actions. Microbial Limit Tests Purpose: Determine the number and types of microorganisms present in non-sterile products. Methods: Total Viable Count (TVC) Aerobic Plate Count: Determines the total number of aerobic bacteria. Yeast and Mold Count: Uses selective media to … Read more
In a pharmaceutical context, Estimation of Ephedrine HCl concentration of in formulations is crucial for quality control and ensuring therapeutic efficacy while maintaining safety. Ephedrine HCl is a medication and stimulant often used in pharmaceutical preparations for its bronchodilating, decongesting, and central nervous system stimulating effects. One of the methods for estimating Ephedrine HCl is … Read more
Acidimetry and alkalimetry are two common types of titrations used to determine the concentration of acidic or basic solutions, respectively. Acidimetry and alkalimetry involve the use of an acid or a base as the titrant to react with the analyte, which is either a base or an acid, respectively. Acidimetry: Acidimetry is a titration process … Read more
Solvents play a crucial role in non-aqueous titrations, as they can influence the solubility of the analyte and titrant, the reaction kinetics, and the acid-base properties of the substances involved. The choice of solvent depends on the specific requirements of the titration and the nature of the analyte and titrant. Here are some common solvent … Read more
Estimation of Sodium Benzoate is important to ensure product safety and compliance with regulations. Sodium benzoate is a common preservative used in food, medications, and cosmetics. The quantitative determination of sodium benzoate can be conducted using various methods, including UV spectroscopy, high-performance liquid chromatography (HPLC), and titration. Typical titration method for estimation of sodium benzoate … Read more
Non-aqueous titration is a type of titration in which the solvent used is not water, but rather a different solvent that does not contain water. They are used when the analyte or the titrant is insoluble in water, when the reaction is too slow in water, or when water-sensitive compounds are involved in the titration. … Read more
Neutralization curves are graphical representation of the pH of a solution during an acid-base titration. The pH is plotted on the y-axis, while the volume of the titrant (either acid or base) added is plotted on the x-axis. The shape of the neutralization curves depends on the nature of the acid and the base involved … Read more
