Enzyme Kinetics: Michaelis-Menten Plot

• Enzyme kinetics is the study of the rates of enzyme-catalyzed reactions.
• One of the most fundamental models in enzyme kinetics is the Michaelis-Menten model, which describes how the reaction rate depends on the concentration of substrate and the enzyme’s affinity for the substrate.

Michaelis-Menten Equation of Enzyme Kinetics

• The Michaelis-Menten equation is given by:
• $v = \frac{V_{\text{max}} [S]}{K_m + [S]} $
  here:
  • 𝑣 is the initial reaction velocity.
  • 𝑉max​ is the maximum reaction velocity.
  • [𝑆] is the substrate concentration.
  • 𝐾𝑚​ is the Michaelis constant, representing the substrate concentration at which the reaction velocity is half of 𝑉max​.

Example Plot

• Here’s an example of how a Michaelis-Menten plot looks:
  1. Hyperbolic Curve: Shows the relationship between [𝑆] and 𝑣.
  2. Key Points:
     1. At low [𝑆], 𝑣 increases linearly.
     2. At high [𝑆], 𝑣 plateaus at 𝑉max​.

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