- Glycosides & its Identification Tests involve detecting sugar-linked compounds with therapeutic significance.
- Glycosides & its Identification Tests include Keller–Killiani, Borntrager’s, and Legal’s test for confirmation.
Definition
- Glycosides are compounds that consist of a sugar (glycone) and a non-sugar (aglycone or genin) part, linked by a glycosidic bond.
Advertisements
Examples
- Cardiac glycosides (e.g., Digoxin from Digitalis purpurea)
- Anthraquinone glycosides (e.g., Senna from Cassia angustifolia)
- Saponin glycosides (e.g., Diosgenin from Dioscorea species)
Properties of Glycosides & its Identification Test
- Bitter or astringent taste.
- Some glycosides are water-soluble.
- Hydrolyzed by acids or enzymes into sugar and aglycone.
Advertisements
Tests for Identification:
-
Keller-Killiani Test (for Cardiac Glycosides):
- Reagent: Ferric chloride and concentrated sulfuric acid.
- Positive Result: Reddish-brown ring at the interface.
-
Borntrager’s Test (for Anthraquinone Glycosides):
- Reagent: Ammonia or sodium hydroxide.
- Positive Result: Pink to red color in the alkaline layer.
-
Foam Test (for Saponin Glycosides):
- Shake the extract with water.
- Positive Result: Persistent frothing indicates saponins.
Advertisements
Thank you for reading from Firsthope's notes, don't forget to check YouTube videos!
