- Growth Conditions in plant tissue culture involve light, temperature, humidity, and sterile environment control.
- Growth Conditions in plant tissue culture ensure optimal cell division, differentiation, and healthy plantlet development.
Sterile Environment
- All culture media, instruments, and vessels must be sterilized (commonly by autoclaving at 121°C, 15 psi, 15–20 min).
- Explants surface-sterilized (e.g., with sodium hypochlorite or ethanol) to remove contaminants.
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Temperature
- Most tissue cultures thrive at 25–28°C.
- Some species require slightly different temperatures for optimal growth.
Light
- Photoperiod typically: 16-hour light / 8-hour dark cycle.
- Light intensity and quality (fluorescent lamps, LEDs) can influence morphogenesis (shoot vs. root induction).
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Humidity
- High relative humidity (>70
- Prevents desiccation of exposed tissues and supports healthy plantlet growth.
pH
- Typically adjusted to 6–5.8 before autoclaving.
- Ensures nutrient availability and stability of the gel matrix.
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