- Historical Development of Plant Tissue Culture traces key milestones from discovery to modern applications in plant science.
- Historical Development of Plant Tissue Culture highlights advances enabling crop improvement and conservation.
- Plant tissue culture has evolved over more than a century, with key contributions from various scientists. Here’s a concise historical timeline:
| Period | Key Developments | Scientists Involved |
| 19th Century | Cell Theory – Cells are the basic units of life. | Schleiden & Schwann (1838-1839) |
| Totipotency Concept – Plant cells can regenerate into whole plants. | Gottlieb Haberlandt (1885) | |
| Early 20th Century | First Tissue Culture Attempts – Unsuccessful due to lack of growth regulators. | Haberlandt (1902) |
| Root Culture – Root tips successfully grown in sterile conditions. | Kotte & Robbins (1922) | |
| Tomato Root Culture – Proved indefinite tissue growth under controlled conditions. | Philip White (1934) | |
| 1930s-1940s | Discovery of Auxin (IAA) – Regulates cell elongation and differentiation. | F.W. Went (1937) |
| Callus Culture – Established using auxins. | White & Nobécourt (1941) | |
| Coconut Milk in Tissue Culture – Found to enhance cell division and shoot regeneration. | Overbeek (1947) | |
| 1950s-1980s | MS Medium – A widely used culture medium. | Murashige & Skoog (1954) |
| Role of Cytokinins – Essential for shoot regeneration. | Skoog & Miller (1962) | |
| Somatic Hybridization – Protoplast fusion techniques for genetic modification. | Various scientists (1970s) | |
| Commercial Applications – Used in agriculture, horticulture, forestry, and pharmaceuticals. | 1980s-Present |
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