Lineweaver-Burk Plot Enzyme Kinetics

• The Lineweaver-Burk plot, also known as the double-reciprocal plot, is a graphical representation used to determine important kinetic parameters of an enzyme-catalyzed reaction, specifically the Michaelis constant (𝐾𝑚​) and the maximum reaction velocity (𝑉max​).

Lineweaver-Burk Equation

$\frac{1}{v} = \frac{K_m}{V_{\text{max}} [S]} + \frac{1}{V_{\text{max}}}$

Description:

• The Lineweaver-Burk plot, or the double reciprocal plot, graphs 1/v​ (reciprocal of the reaction rate) against 1/[S]​ (reciprocal of the substrate concentration).
• This transformation facilitates a linear relationship that simplifies the determination of Vmax​ and Km​.
• In this plot:
  • The y-intercept equals 1/Vmax​​.
  • The x-intercept is −1/−Km​​.
  • The slope represents Vmax/​Km​​.

Advantages and Uses:

• The linear nature of allows for a straightforward extraction of kinetic parameters. Additionally, this plot is particularly valuable in enzyme inhibition studies.
• By comparing changes in the plot’s slope, y-intercept, and x-intercept, one can deduce the type of inhibition (competitive, non-competitive, or uncompetitive) affecting the enzyme.

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