- Types of Bioassay: Include quantal, graded, and endpoint assays to measure drug effects.
- Types of Bioassay: Used to determine potency, efficacy, and standardization of drugs.
- Bioassays can be broadly classified based on the nature of the biological system used and the type of response measured.
Types of Bioassay
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Graded Response Bioassay
- Measures the intensity of a response (e.g., smooth muscle contraction, blood pressure change) with increasing doses.
- A classic example is the analytical method on isolated organ bath (e.g., guinea pig ileum for histamine).
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Quantal (All-or-None) Response Bioassay:
- A yes-or-no response (e.g., lethal or non-lethal in animal studies).
- Typically used in toxicity studies (LD50).
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Matching Method or Direct Bioassay
- Compare the response produced by a single known dose of standard with that of the test sample until you find a dose of the test that matches the standard’s response.
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Interpolation Methods
- Construct a dose-response curve for the standard and read off the test sample’s potency from the same curve.
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